产品简介：

    pCMV-C-Myc是上海华潍自行研发的用于在哺乳动物细胞中表达C端和Myc tag(Myc标签)融合的目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达；在多克隆位点的3'端含有一个可以编码Myc标签的序列，因此可以表达出含有Myc标签的融合蛋白，可以方便地使用抗Myc的抗体来识别目的蛋白，有利于目的蛋白检测和分离纯化。质粒为卡那霉素抗性。转染细胞后，可使用G418筛选稳定表达目的蛋白的细胞株。
    pCMV-C-Myc质粒的主要信息如下：
    Feature Nucleotide                                             Position
    CMV promoter                                                   1–602
    T3 promoter and T3 primer binding site                         620–639
    multiple cloning site                                          680-740
    c-Myc tag                                                      741-770
    T7 promoter and T7 primer binding site                         823–844
    SV40 polyA signal                                              856–1239
    f1 origin of ss-DNA replication                                1377–1681
    bla promoter                                                   1706–1830
    SV40 promoter                                                  1850–2188
    neomycin/kanamycin resistance ORF                              2223–3014
    HSV-thymidine kinase(TK)polyA signal                           3015–3473
    pUC origin                                                     3602–4269
      pCMV-C-Myc的多克隆位点的详细图谱如下：
                                       XmaI                 PstI
        SacI                           SmaI    BamHI  HindIII
    651 GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCAAGCTTCT
        CTCGAGGTGG CGCCACCGCC GGCGAGATCG GGCCCGCCTA GGTTCGAAGA
                                                           Myc
            EcoRI  EcoRV SalI   BglII XhoI   XbaI   E  Q  K  L
    701 GCAGGAATTC GATATCGTCG ACAGATCTCT CGAGTCTAGA GAGCAGAAAC
        CGTCCTTAAG CTATAGCAGC TGTCTAGAGA GCTCAGATCT CTCGTCTTTG
        tag                  
          I  S  E   E  D  L      ApaI 
    751 TCATCTCTGA AGAGGATCTG TAAGGGCCCG GTACCTTAAT TAATTAAGGT
        AGTAGAGACT TCTCCTAGAC ATTCCCGGGC CATGGAATTA ATTAATTCCA
    pCMV-C-Myc中没有的酶切位点(Restriction enzymes that do not cut pCMV-C-Myc)包括：
    Afl II    Age I       Ahd I     Asc I     Bbs I       Bbv II    Blp I
    Bsg I     BsiW I      BsmB I    BspM II   BsrG I      BssH II   Bst1107 I
    BstE II   Eco47 III   Eco72 I   EcoN I    Esp I       Fse I     Nru I
    PflM I    Pme I       Pml I     PpuM I    Psp1406 I   Sap I     Sca I
    Spe I     Spl I
    pCMV-C-Myc中的单酶切位点(Restriction enzymes that cut pCMV-C-Myc once)包括：

    pCMV-C-Myc质粒中对于插入片断进行测序时，推荐使用的正向测序引物T3和反向测序引物T7的序列如下：
    T3 primer(620–639): 5′AATTAACCCTCACTAAAGGG 3′
    T7 primer(823-844): 5′GTAATACGACTCACTATAGGGC 3′
    pCMV-C-Myc的全序列信息： PHWE4449 pCMV-C-Myc-seq.txt